Coding

Part:BBa_K559001

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM11_Hong_Kong-CUHK   (2011-09-29)

Halorhodopsin under T7 promoter

Halorhodopsin under constitute T7 promoter to regulate the gene transcription to be maximized and no restrict to other regulatory factor

Use of T7 promoter provides an unlimited maximized expression of the downstream halorhodopsin gene, so that the halorhodopsin light-driven ion pump will not be a limiting factor in the light sensing system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 368
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 380
    Illegal AgeI site found at 106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 316


[edit]
Categories
//cds/membrane/channel
//chassis/prokaryote/ecoli
//function/sensor/light
//plasmid/expression/t7
Parameters
biology
function
protein